Classification And Discussion Of Esterification - 1017 Words

Results and discussion Esterification extent: The color reaction using hydroxylamine hydrochloride was used with modification to quantify the extent of esterification of proteins and the results showed that the highest level of esterification was recorded for esterified cowpea protein isolate (82%) followed by esterified common bean protein isolate (79%). Functional properties of native and modified proteins: PH-solubility profile: The pH-solubility curves of native and esterified cowpea protein are shown in Fig.1. The solubility profile of native cowpea indicate that protein solubility reduced as the pH increased from 2 to 4, which corresponding to its isoelectric point, after which subsequent increases in pH increased protein solubility progressively. The minimum solubility for native cowpea (10 %) was at pH 4 which corresponds to its isoelectric point ( IP).The highest protein solubility (80%) was observed at pH 10. Esterification increased protein solubility in the acidic pH range from 2 to 5. Increasing pH more than 5 reduced the solubility and giving a minimum value (9 %) at pH 6. Methylated cowpea protein was more soluble in the acidic range of pH and less soluble in the alkaline range of pH as compared to unmodified protein. On the other hand The solubility profile of native common bean indicate that protein solubility reduced as the pH increased from 2 to 5, which corresponding to its isoelectric point , after which subsequent increases in pH increased proteinShow MoreRelatedOrganic Chemistry Experiment 10 Formal Report1855 Words   |  8 PagesOrganic Chemistry Laboratory – CH 200L (2012 – 2013) 2B-Ph Group 9 Experiment 10 Identifying Carboxylic Acids and Derivatives Through Classification Tests Bianca Therese Rivera, Camille Aliana Rivera, Zarah Mae Roxas, Ma. Rosario Teresa Saylo, Jean Darlene Semilla and Adrian Yu Department of Pharmacy, Faculty of Pharmacy University of Santo Tomas, Espana Street, Manila 1008 Date Submitted: September 13, 2012 ------------------------------------------------- Abstract Carboxylic acid derivativesRead MoreBiodiesel As A Alternative Fuel Source5479 Words   |  22 PagesCHAPTER I INTRODUCTION 1.1 BACKGROUND Biodiesel is produced from vegetable oils, used cooking oils, and animal fats by the production process called â€Å"trans-esterification† to convert those mixtures to the chemicals which have long chain mono alkyl esters or fatty acid methyl esters which can be used as â€Å"fuel† for vehicles. Approximately, 100 pounds of vegetable oil react with 10 pounds of alcohol (commonly methanol) in the presence of a catalyst, sodium hydroxide; later, 100 pounds of biodiesel